Bio Rad Automated Droplet Generator PCR Thermal Cycler Description

Item#: 28907
Tag: PCR / Thermal Cycler

Description

Droplet Digital PCR (ddPCR) is a digital polymerase chain reaction method based on water-oil emulsion droplet technology. ddPCR uses a combination of microfluidics and proprietary surfactant chemistries to divide each sample into water-in-oil droplets. The technology uses reagents and workflows similar to those used for most standard TaqMan probe-based assays, and provides absolute quantification of nucleic acid target sequences by counting nucleic acid molecules encapsulated in discrete, volumetrically defined water-in-oil droplet partitions.

Features and Benefits: 

Bio-Rad’s Automated Droplet Generator simplifies ddPCR workflow and makes digital PCR scalable and flexible:

• Guides setup and loading with large, color touch-screen interface and LED lighting
• Generates droplets for 96 ddPCR reactions in under 45 minutes
• One AutoDG Instrument can generate sufficient droplets to keep 4–5 droplet readers running continuously
• Provides operational flexibility:
  • • • User-definable plate layout with increments of 8 wells
      • HEPA-filtered enclosure reduces the possibility of contamination and allows the system to be placed in any lab environment
      • Compatible with both QX600 and QX200 ddPCR workflows

Specifications: 

• Weight: 100 lb (45.4 kg)
• Size (W x D x H): 26 x 22 x 26 in (66 x 56 x 66 cm)
• Automated Droplet Generator: Instrument used to generate standard droplets
• Cooling block accessory: Prevents evaporation during droplet generation
• Power cord: U.S. standard power cord
• Oil purge reservoir: Collects oil waste from priming and flushing
• ddPCR 96-Well Plates: Clear well/clear shell semi-skirted plates 
• DG32 Droplet Generation Cartridges: Microfluidic cartridge with gasket for the AutoDG
• Automated Droplet Generation Oils:

• • • Automated droplet generation oils for the AutoDG
    • Automated Droplet Generation Oil for Probes
    • Automated Droplet Generation Oil for EvaGreen

• Droplet Reader Oil: ddPCR droplet reader oil for QX600 and QX200 instruments and systems
• Pipet Tips: Pipet tips for the AutoDG
• Pipet Tip Bins: Pipet tip waste bins for the AutoDG