Forte Bio Octet Red96e

Item#: 28716
Tag: SPR Analyzer

Description

ForteBio’s Octet RED96e system is a multi-functional, label-free, real-time analysis instrument. It is ideal for rapidly measuring concentration of proteins and other biomolecules, measuring kinetics and affinity. The Octet RED96e system is a 96-well characterization of protein-protein and protein-small molecule binding kinetics, and for the determination of protein concentrations and titer. Popular industrial applications include expression screening, on- and off-rate screening, epitope binning, cell line development and bioprocess optimization.

Specifications:

• Detection technology: Bio-Layer Interferometry (BLI)
• Biosensor type: Disposable, single-use fiber optic biosensors with optional reuse by regeneration and/or re-racking in the sensor tray
• Information provided: 
  • Yes/No binding
  • Kinetic and affinity analysis (kobs, ka, kd, KD)
  • Specific and selective detection of molecules, even in crude samples
  • Relative and absolute quantitation of specific proteins in crude matrices or purified samples
• Data presentation:
  • Plots displaying real-time kinetic binding sensorgrams, fitted result plots, and residuals of fits
  • Concentration data analysis including calibration curves and output of tabulated concentration data
  • Tabulated kinetic data
  • Epitope binning and cross-blocking matrices and trace overlays
• Sample types: Proteins, antibodies, peptides, DNA, RNA,
  liposomes, bacterial cells, viruses, mammalian cells, small molecules in various media including serum, buffers containing DMSO, periplasmic fractions, untreated cell culture supernatants, and crude cell lysates
• Number of spectrometers: 8
• Maximum simultaneous reads: 8
• Data collection rate: 2, 5, or 10 Hz
• Sample position and format: 1 standard 96-well, black, flat bottom microplate
• Sample volume: 180–220 μL/well, non-destructive testing
• Orbital flow capacity: Static or 100–1500 rpm
• Analysis temperature range: 15–40°C, 1°C increments
• Workflow: Up to 8 assays in parallel; up to 96 assays
  per 96-well plate
• Molecular weight detection: >150 Da
• Analysis time per sample: Real-time kinetic binding experiments from 5 minutes to 4 hours, or up to 12 hours with
  the evaporation cover
• Association rate constant (k2): 101-107 M-1s-1
• Dissociation rate constant (k2): 10-6-0.1 s-1
• Affinity (KD) constant: 1 mM-10 pM
• Baseline noise: ≤ 3.5 pm (RMS)
• Baseline drift: ≤ 0.1 nm/hour
• Workflow: Up to 8 assays in parallel; up to 96 assays per 96-well plate
• Analysis time per sample: Human IgG quantitation in 2 minutes for 8 samples, ≤ 32 minutes for 96 samples
• Direct quantitation range for human IgG with Protein A Biosensor: 0.05-2000 ug/mL
• Dimensions (H x W x D): 19.5 in x 22 in x 18.2 in (49 cm x 56 cm x 46 cm)
• Weight:  72 lb (32.7 Kg)
• Electrical requirements: Mains: 100-120/200-240 VAC, 50/60 Hz, 4 A max
• Power consumption: 200 W (300 W peak)
• PC operating systems: Windows® 10 Professional, 64-bit, Windows 7 Professional, 64-bit, Windows 7 Professional, 32-bit
• Safety standards: CE, Nemko